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Image Search Results
Journal:
Article Title: The Condensin I Subunit Barren/CAP-H Is Essential for the Structural Integrity of Centromeric Heterochromatin during Mitosis
doi: 10.1128/MCB.25.20.8971-8984.2005
Figure Lengend Snippet: Immunolocalization of dimethylated K9 histone H3 in the heterochromatin of Barren/CAP-H RNAi-depleted chromosomes. Control and Barren/CAP-H cells were immunostained for CID and K9 dimethylated histone H3 (diMeK9). (A) In control cells at metaphase, diMeK9 is confined to heterochromatin. However, after depletion of Barren/CAP-H the pattern of diMeK9 appears to be significantly altered under bipolar attachment. diMeK9 is now detected over a broad area of chromatin localized between the two CID-labeled centromeres. (B) Both control and Barren-CAP-H-depleted cells were incubated with 30 μM colchicine for 2 h to depolymerize microtubules and the pattern of diMeK9 was determined. Note that under these conditions diMeK9 is confined to a tight region between sister centromeres in both control and Barren/CAP-H-depleted cells. Scale bars are 5 μm.
Article Snippet: The primary antibodies were anti-α-tubulin mouse B512 (Sigma-Aldrich) used at 1:4,000 for immunofluorescence and 1:10,000 for immunoblotting; anti-phospho-histone H3 rabbit polyclonal (Upstate Biotechnology) used at 1:1,000; anti-POLO mouse monoclonal MA294 ( 26 ) used at 1:30; anti-Barren/CAP-H rabbit polyclonal ( 4 ) used at 1:1,500 (immunofluorescence) and 1:3,000 (immunoblotting); anti-DmSMC4 ( 40 ) rabbit polyclonal used at 1:500 (immunoblotting) and sheep polyclonal used at 1:500 (immunofluorescence); anti-SMC2 rabbit polyclonal used at 1:1,000; anti-CAP-D2 rabbit polyclonal used at 1:10,000 (immunoblotting) and 1:2,000 (immunofluorescence); immunopurified anti-CAP-G rabbit polyclonal used at 1:5; anti-CID chicken polyclonal ( 6 ) used at 1:100; anti-CID rabbit polyclonal ( 15 ) used at 1:1,500;
Techniques: Labeling, Incubation